Biosafety Level 3 is applicable to clinical, diagnostic, teaching, research, or production facilities in which work is done with indigenous or exotic agents which may cause serious or potentially lethal disease as a result of exposure by the inhalation route. Laboratory personnel have specific training in handling pathogenic and potentially lethal agents, and are supervised by competent scientists who are experienced in working with these agents.

A Biosafety-3 laboratory is under construction on the Shelby Campus in the eastern corner contiguous to Hurstbourne Lane. The laboratory is expected to be completed by 2009.